Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 123241..123968 Replicon plasmid pS161-2.2
Accession NZ_CP058546
Organism Klebsiella pneumoniae strain S161-2

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag HYH18_RS27455 Protein ID WP_011251285.1
Coordinates 123241..123552 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HYH18_RS27460 Protein ID WP_011251286.1
Coordinates 123549..123968 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH18_RS27420 118410..118508 - 99 WP_004118688.1 type II toxin-antitoxin system RelE/ParE family toxin -
HYH18_RS27425 118656..119647 + 992 Protein_134 IS3 family transposase -
HYH18_RS27430 119679..120647 + 969 WP_194433525.1 IS5 family transposase -
HYH18_RS27435 120705..121754 - 1050 Protein_136 IS66 family transposase -
HYH18_RS27440 121803..122150 - 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
HYH18_RS27445 122147..122485 - 339 WP_023288266.1 transposase -
HYH18_RS27450 122599..123036 + 438 Protein_139 DDE-type integrase/transposase/recombinase -
HYH18_RS27455 123241..123552 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
HYH18_RS27460 123549..123968 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
HYH18_RS27465 124115..125083 + 969 WP_194433524.1 IS5 family transposase -
HYH18_RS27470 125337..126125 - 789 WP_114263119.1 hypothetical protein -
HYH18_RS27475 126125..126421 - 297 WP_227662480.1 conjugative transfer protein MobI(A/C) -
HYH18_RS27480 127182..127817 + 636 WP_227662472.1 hypothetical protein -
HYH18_RS27485 128130..128654 + 525 WP_071444207.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / blaDHA-1 / qnrB4 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..178411 178411
- inside IScluster/Tn - - 118629..143865 25236


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T166772 WP_011251285.1 NZ_CP058546:123241-123552 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T166772 NZ_CP074428:c4031797-4031489 [Salmonella sp. SJTUF14523]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT166772 WP_011251286.1 NZ_CP058546:123549-123968 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT166772 NZ_CP074428:c4032039-4031800 [Salmonella sp. SJTUF14523]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References