Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4526896..4527493 Replicon chromosome
Accession NZ_CP058544
Organism Klebsiella pneumoniae strain S161-2

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HYH18_RS22305 Protein ID WP_004142563.1
Coordinates 4527176..4527493 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HYH18_RS22300 Protein ID WP_004142561.1
Coordinates 4526896..4527183 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH18_RS22270 4522976..4523224 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HYH18_RS22275 4523242..4523583 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HYH18_RS22280 4523614..4524729 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
HYH18_RS22285 4524909..4525493 + 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
HYH18_RS22290 4525490..4525858 + 369 WP_237085225.1 MmcQ/YjbR family DNA-binding protein -
HYH18_RS22295 4525978..4526631 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HYH18_RS22300 4526896..4527183 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HYH18_RS22305 4527176..4527493 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HYH18_RS22310 4527678..4528721 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
HYH18_RS22315 4529391..4530257 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HYH18_RS22320 4530366..4531793 + 1428 WP_064159319.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T166768 WP_004142563.1 NZ_CP058544:c4527493-4527176 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T166768 NZ_CP074428:c2693594-2693491 [Salmonella sp. SJTUF14523]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT166768 WP_004142561.1 NZ_CP058544:c4527183-4526896 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT166768 NZ_CP074428:2693489-2693634 [Salmonella sp. SJTUF14523]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References