Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324762..325348 Replicon chromosome
Accession NZ_CP058358
Organism Klebsiella pneumoniae strain KP486

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag HYD35_RS01510 Protein ID WP_002920800.1
Coordinates 324980..325348 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HYD35_RS01505 Protein ID WP_004174006.1
Coordinates 324762..324983 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD35_RS01485 (320919) 320919..321845 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HYD35_RS01490 (321842) 321842..323119 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
HYD35_RS01495 (323116) 323116..323883 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HYD35_RS01500 (323885) 323885..324598 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HYD35_RS01505 (324762) 324762..324983 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HYD35_RS01510 (324980) 324980..325348 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HYD35_RS01515 (325621) 325621..326937 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HYD35_RS01520 (327044) 327044..327931 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HYD35_RS01525 (327928) 327928..328773 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HYD35_RS01530 (328775) 328775..329845 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 319748..330582 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T166700 WP_002920800.1 NZ_CP058358:324980-325348 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T166700 NZ_CP074405:c3744386-3743994 [Cellulomonas wangleii]
GTGGCGTACTACCTCGACACCTCCGCCCTGGTGAAGCTGGTCGTCGACGAGGGGGAGACGGCGGCACTGCGGGAGTGGTG
GCGGTCGCACGGCACCACTCCCGTCTCGTGCGACCTGGCGCGAACCGAGCTGATGCGCGCCGTCCGCAGGGCCGCACCCG
AGGCCGCAGTGCAGGTACACGCGGTGCTCGATGCGCTTGTGCTGGTGTCCGTGACCGCGCGAGTCTTCGACGCGGCCGGG
CGGTTGGAGCCGACGACACTGCGGTCGCTCGACGCGATCCACCTGGCTGCCGCTCTCGAGCTCGGCGACGACCTCGAGGG
GATCGTGACCTACGACGAGCGGCTCGCCGAGGCGGCGACGTCGTACGGCGTCCCCGTGGTCGCGCCCGCCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT166700 WP_004174006.1 NZ_CP058358:324762-324983 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT166700 NZ_CP074405:c3744661-3744389 [Cellulomonas wangleii]
ATGGGCACGATCGGCATTCGCGAGCTCAAGCAGAACGCGTCTGCGGCGGTCGCGCGCGCGGCGGCCGGTGAGACGCTGAC
CGTGACCGATCGCGGACGGCCGGCGGCACAGCTGTCGCCACTGCCGACGTCGCCCCTGGCGCGTCTGCTCGAGAGCGGGT
CCGCGCGTCCGGCACGCACCGACCTCGCCGACCTCCCTTCCCCCGTTCGCGCAGCGGGCGGGCGATCCGCGCTGTCCGAC
GCGCTCGCCGACCTGCGCTCCGACGAGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References