Detailed information of TA system TA166696

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84474..84900
• Replicon: plasmid pAMPD2
• Accession: NZ_CP058357
• Organism: Escherichia coli strain AMSCJX03

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HXS77_RS23180
• Protein ID: WP_001312861.1
• Coordinates: 84474..84632 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	84676..84900 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS77_RS23150	79524..79751	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
HXS77_RS23155	79845..80531	-	687	WP_000332484.1	PAS domain-containing protein	-
HXS77_RS23160	80722..81105	-	384	WP_000124981.1	relaxosome protein TraM	-
HXS77_RS23165	81382..82029	+	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
HXS77_RS23170	82326..83147	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HXS77_RS23175	83265..83552	-	288	WP_000107544.1	hypothetical protein	-
HXS77_RS23180	84474..84632	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	84676..84900	-	225	NuclAT_0	-	Antitoxin
-	84676..84900	-	225	NuclAT_0	-	Antitoxin
-	84676..84900	-	225	NuclAT_0	-	Antitoxin
-	84676..84900	-	225	NuclAT_0	-	Antitoxin
HXS77_RS23185	84712..84900	+	189	WP_001299721.1	hypothetical protein	-
HXS77_RS23190	84912..85631	-	720	WP_013362833.1	plasmid SOS inhibition protein A	-
HXS77_RS23195	85628..86062	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HXS77_RS23200	86117..88075	-	1959	WP_065800285.1	ParB/RepB/Spo0J family partition protein	-
HXS77_RS23205	88141..88374	-	234	WP_000005990.1	DUF905 family protein	-
HXS77_RS23210	88437..88976	-	540	WP_001825195.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-55	-	1..88999	88999

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166696 WP_001312861.1 NZ_CP058357:c84632-84474 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166696 NZ_CP074400:c484456-484160 [Agrobacterium salinitolerans]
ATGCGCTATGTTCTTTCTGCTGAGGCGGAAGAAGACATCATCGGAATCGCTGAGTATGGGGTCCGCAACTTCGGCACAAC
TCATGCGCGCCGTTACCACAATGAGCTGTTCGAGACGCTCGATCTAATTGCCCGCCACCCCGGGATGGCGAGAGAGCGCC
GCGAAATCCAACCGCCCGTGCGTATCCACCCGTTCAAGGCACATCTCATAGTCTACGTACTCGGTGAAGATGGCGTAGTG
TTCGTCATCCGCGTCCGCCACGGCCACGAGGACTGGGCTAGCAGCGACGACGATTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT166696 NZ_CP058357:c84900-84676 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin