Detailed information of TA system TA166692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1026..1452
• Replicon: plasmid pAMPD2
• Accession: NZ_CP058357
• Organism: Escherichia coli strain AMSCJX03

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HXS77_RS22715
• Protein ID: WP_001312861.1
• Coordinates: 1026..1184 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1228..1452 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS77_RS22715	1026..1184	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	1228..1452	-	225	NuclAT_1	-	-
-	1228..1452	-	225	NuclAT_1	-	-
-	1228..1452	-	225	NuclAT_1	-	-
-	1228..1452	-	225	NuclAT_1	-	-
HXS77_RS22720	1421..2182	-	762	Protein_1	plasmid SOS inhibition protein A	-
HXS77_RS22725	2179..2613	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HXS77_RS22730	2668..4628	-	1961	Protein_3	ParB/RepB/Spo0J family partition protein	-
HXS77_RS22735	4695..4928	-	234	Protein_4	DUF905 family protein	-
HXS77_RS22740	4991..5444	-	454	Protein_5	single-stranded DNA-binding protein	-
HXS77_RS22745	6174..6401	-	228	WP_001825119.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-55	-	1..88999	88999

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166692 WP_001312861.1 NZ_CP058357:c1184-1026 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166692 NZ_CP074393:602438-602797 [Agrobacterium salinitolerans]
ATGGTCCGCAGTCAGGTTCCAAAACGTGGCGACGTTTATCTGGTCGACCTCAATCCTGTGATCGGCAGCGAAATCAGGGA
CGAACATCGTTGTATCGTCATCACGCCCAGAGAAATCAATGCGGTTGGACTGTGCCTTGTCGTTCCCGTGACCACGGGCG
GACTATTCACGCGCAGGGCGGGGCTTGCCGTGAACATATCGGGCCATAAGACAACCGGTGTCGCACTATGCAACCAGGTG
AGAAGCATGGACATTGTTGCCCGTGCCGCCCAGAAGAAAGCGAAATATATCGAAACCCTTGACGATGCGACGATCGACGA
AATCGCCGGTCGTGTCATCAGCATGATCGATCCCGCCTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT166692 NZ_CP058357:1228-1452 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGCAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTCCCTGAAAATAATCCGCTCATTCAGACCGTTCACGGGAAATCCGTGTGA

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin