Detailed information of TA system TA166689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3603009..3603703
• Replicon: chromosome
• Accession: NZ_CP058355
• Organism: Escherichia coli strain AMSCJX03

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HXS77_RS17380
• Protein ID: WP_001263493.1
• Coordinates: 3603009..3603407 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HXS77_RS17385
• Protein ID: WP_000554757.1
• Coordinates: 3603410..3603703 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS77_RS17350	3598009..3599253	-	1245	WP_000189541.1	esterase FrsA	-
-	3598669..3598749	-	81	NuclAT_8	-	-
-	3598669..3598749	-	81	NuclAT_8	-	-
-	3598669..3598749	-	81	NuclAT_8	-	-
-	3598669..3598749	-	81	NuclAT_8	-	-
HXS77_RS17355	3599345..3599803	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HXS77_RS17360	3600064..3601521	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
HXS77_RS17365	3601578..3602099	-	522	Protein_3396	peptide chain release factor H	-
HXS77_RS17370	3602098..3602301	-	204	Protein_3397	RNA ligase RtcB family protein	-
HXS77_RS17375	3602547..3602999	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HXS77_RS17380	3603009..3603407	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HXS77_RS17385	3603410..3603703	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HXS77_RS17390	3603755..3604810	-	1056	WP_001226164.1	DNA polymerase IV	-
HXS77_RS17395	3604881..3605804	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
HXS77_RS17400	3605807..3606286	-	480	Protein_3403	MotA/TolQ/ExbB proton channel family protein	-
HXS77_RS17405	3606301..3606998	-	698	Protein_3404	IS1 family transposase	-
HXS77_RS17410	3607207..3607947	+	741	WP_001225679.1	murein L,D-transpeptidase	-
HXS77_RS17415	3607918..3608685	-	768	WP_000333380.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T166689 WP_001263493.1 NZ_CP058355:c3603407-3603009 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T166689 NZ_CP074392:c563213-562932 [Agrobacterium salinitolerans]
ATGATCCGCTCTTTCAAAAACAAGCTGACTGCGTCGATTGACGACGGATCTATGAAGAAGGGCTTTCCCGCCGATCTGGT
ACGCCGCGCCCAGCAGCTGCTCACGATCCTGGACGCGGCGACCACGGTAGAAGACTTTCGGTCACCGCCCGGTAACCGTT
TGGAAAAGCTGTCCGGCGACCGTGAAGGCCAATATTCGATCCGGATCAACAAGCAGTGGCGCATCTGCTTCGTCTGGACG
GAAGCAGGGCCGGAAAACGTGGAAATAACCGACTATCACTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT166689 WP_000554757.1 NZ_CP058355:c3603703-3603410 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT166689 NZ_CP074392:c562875-562576 [Agrobacterium salinitolerans]
ATGACAATCAGTAAATCGCCTCCGATCCATCCGGGGGAAATCCTGCGGGAACTCTATCTTGAGCCGCTGGGGATGTCTGC
TTACGCTCTTGCAAAGCACCTCAATATTCCGCGCACCCGCATAGAACGCATTGCCGGAGAGAAACTCGGTATCACCGCCG
ACACCGCCTTGCGCCTGGCAAAATTTTTCAAGACCACACCGGAATATTGGCTGAACCTGCAGAACGCCTTTGACATTAAG
GCGGCGGCTGCGAGGCTGGAAGCAGACCTTGCCAATATCAAAGAACTGGAAGCGGCCTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6