Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1762342..1762844 Replicon chromosome
Accession NZ_CP058355
Organism Escherichia coli strain AMSCJX03

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HXS77_RS08305 Protein ID WP_000767829.1
Coordinates 1762590..1762844 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HXS77_RS08300 Protein ID WP_001259255.1
Coordinates 1762342..1762593 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS77_RS08275 1757521..1758588 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HXS77_RS08280 1758588..1759658 - 1071 WP_178887394.1 histidinol-phosphate transaminase -
HXS77_RS08285 1759655..1760959 - 1305 WP_029399146.1 histidinol dehydrogenase -
HXS77_RS08290 1760965..1761864 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HXS77_RS08295 1762010..1762060 - 51 WP_001364200.1 his operon leader peptide -
HXS77_RS08300 1762342..1762593 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HXS77_RS08305 1762590..1762844 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HXS77_RS08310 1762927..1763751 + 825 WP_029399144.1 SDR family oxidoreductase -
HXS77_RS08315 1763797..1764726 + 930 WP_000803351.1 LysR family transcriptional regulator -
HXS77_RS08320 1764941..1765003 + 63 WP_010723108.1 membrane protein YoeI -
HXS77_RS08325 1764993..1766351 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HXS77_RS08330 1766530..1767588 + 1059 WP_032352489.1 transport protein YeeE -
HXS77_RS08335 1767602..1767829 + 228 WP_097430815.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T166676 WP_000767829.1 NZ_CP058355:1762590-1762844 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T166676 NZ_CP074371:3912462-3912728 [Nocardia tengchongensis]
ATGACCTATGCGGTCGAATTCACCCCAACCGCACGGCGCGAGATCGCCAAGCTGCCGGAGCGGATCGCCTGGGCGGTGCT
CGAATTCTGTTCGGGTCCGCTCGCCGAGAACCCGCATCGAGTGGGAAAACCACTGATCGGTGACCTCGCGGGTCTGCACT
CGGCCCGTCGCGGCGAATACCGGGTCATCTACGCCATCTACGACGAGAGGGTTGTCGTGGAAGTCGCGACGGTGCGTCAT
CGCGGCACCGCCTACCGGCCGAGGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT166676 WP_001259255.1 NZ_CP058355:1762342-1762593 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT166676 NZ_CP074371:3912178-3912465 [Nocardia tengchongensis]
ATGACTACTTGCTTGCCGCTCGCCGAGGTTCGTGACCGTCTATCGCCGCTGGTCAGTTCCGTCCAGACCACGCATGAACG
GGTGGTCATCACCAAGAACGGCCACCCGGCCGCTGTCCTCATCGCCTACGAGGATCTCGAATCCTTGCAGGAGACAGTGG
AGATCCTTGCCGATCCCGGGATCGTCGACGAGATCCGTGCGGCTCTGGCCGAGCCGGAGCGCTTCACCCTCGAGGACATT
CGGAGGGACCTCGAGGCTCGCGGCGGGTCCGAGGACGGTGACAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References