Detailed information of TA system TA166638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 788709..789316
• Replicon: chromosome
• Accession: NZ_CP058349
• Organism: Pseudomonas sp. ABC1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HW090_RS03405
• Protein ID: WP_179112158.1
• Coordinates: 788709..789023 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW090_RS03410
• Protein ID: WP_179112159.1
• Coordinates: 789029..789316 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW090_RS03360	783805..784038	-	234	WP_179112150.1	hypothetical protein	-
HW090_RS03365	784035..784226	-	192	WP_179114809.1	AlpA family phage regulatory protein	-
HW090_RS03370	784387..784590	-	204	WP_179112151.1	helix-turn-helix domain-containing protein	-
HW090_RS03375	784776..785261	+	486	WP_179112152.1	hypothetical protein	-
HW090_RS03380	785385..786233	-	849	WP_179112153.1	hypothetical protein	-
HW090_RS03385	786349..786564	-	216	WP_179112154.1	hypothetical protein	-
HW090_RS03390	786772..787080	+	309	WP_179112155.1	hypothetical protein	-
HW090_RS03395	787587..788159	+	573	WP_179112156.1	DUF4760 domain-containing protein	-
HW090_RS03400	788440..788646	-	207	WP_179112157.1	hypothetical protein	-
HW090_RS03405	788709..789023	+	315	WP_179112158.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW090_RS03410	789029..789316	+	288	WP_179112159.1	helix-turn-helix domain-containing protein	Antitoxin
HW090_RS03415	789618..790703	-	1086	WP_179112160.1	AI-2E family transporter	-
HW090_RS03420	790727..791524	-	798	WP_179114810.1	DUF4892 domain-containing protein	-
HW090_RS03425	791728..792150	-	423	WP_179114811.1	hotdog fold thioesterase	-
HW090_RS03430	792208..792663	-	456	WP_179112161.1	phosphohistidine phosphatase SixA	-
HW090_RS03435	792660..792986	-	327	WP_179112162.1	DUF4389 domain-containing protein	-
HW090_RS03440	792983..794026	-	1044	WP_179112163.1	NAD(P)H-dependent glycerol-3-phosphate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	772160..792150	19990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11925.59 Da
• Isoelectric Point: 9.8035

>T166638 WP_179112158.1 NZ_CP058349:788709-789023 [Pseudomonas sp. ABC1]
MIFIETPIFTKQLRELLDDDSYAAFQRTLADRPEQGDVIEGTGGIRKVRVASSGRGKRGGSRVIYYHFSSASQIALLLIY
PKNEKDDLSTEERKALKHIIERWR

Nucleotide

• Length: 315 bp

>T166638 NZ_CP074364:c2413494-2413075 [Xanthomonas translucens pv. cerealis]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAACCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGCTGAAACCCTCTACCTGTCCAGCGTGACGCTGGCCGAGCTGTTCTTCGGCATCGTCGCGCTGCCGAACGGGA
AACGTAAGAAAGGCTTGGGCGAAGCCCTGGACGGCTTGCTCGAGCTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCCGCTCGCCATTATGCCGAGCTGGCGGTGAAAGCCCGCACAGCCGGGAAGGATTTTCCAACACCCGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTTGAAGCGGCCGGGCTTACTGTTATCA
ATCCTTGGAACCATCGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10780.26 Da
• Isoelectric Point: 7.3213

>AT166638 WP_179112159.1 NZ_CP058349:789029-789316 [Pseudomonas sp. ABC1]
MSKFFDDLLESVQQMDEIHRGERQPSREFTVDALQVKEIRKATGLTQARFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPAHVIQALAN

Nucleotide

• Length: 288 bp

>AT166638 NZ_CP074364:c2413745-2413491 [Xanthomonas translucens pv. cerealis]
ATGGCAATCCTGACAGTGCGGAATGTGCCCGACGAGGTACATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTCCGCGAGATTCTGGAGAGTGCGGTTAAGTCAGAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCGCGTACGGCTAACAAACGATGATTTCGCAGTATTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA