Detailed information of TA system TA166628

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20451..20877
• Replicon: plasmid pECW602
• Accession: NZ_CP058344
• Organism: Escherichia coli strain W60

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HXP40_RS23685
• Protein ID: WP_001312861.1
• Coordinates: 20451..20609 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20653..20877 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXP40_RS23655	15829..16518	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
HXP40_RS23660	16705..17088	-	384	WP_053320747.1	relaxosome protein TraM	-
HXP40_RS23665	17409..18011	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HXP40_RS23670	18308..19129	-	822	WP_001234445.1	DUF945 domain-containing protein	-
HXP40_RS23675	19240..19536	-	297	WP_001272251.1	hypothetical protein	-
HXP40_RS23680	19836..20213	+	378	Protein_27	hypothetical protein	-
HXP40_RS23685	20451..20609	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	20653..20877	-	225	NuclAT_0	-	Antitoxin
-	20653..20877	-	225	NuclAT_0	-	Antitoxin
-	20653..20877	-	225	NuclAT_0	-	Antitoxin
-	20653..20877	-	225	NuclAT_0	-	Antitoxin
HXP40_RS23690	20689..20868	+	180	WP_001309233.1	hypothetical protein	-
HXP40_RS23695	20889..21608	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
HXP40_RS23700	21605..22039	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HXP40_RS23705	22108..24131	-	2024	Protein_32	ParB/RepB/Spo0J family partition protein	-
HXP40_RS23710	24192..24425	-	234	WP_000006003.1	DUF905 family protein	-
HXP40_RS23715	24483..24968	-	486	WP_031311812.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / aph(3')-IIa / floR / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..94776	94776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166628 WP_001312861.1 NZ_CP058344:c20609-20451 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166628 NZ_CP074361:3618411-3618668 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT166628 NZ_CP058344:c20877-20653 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin