Detailed information of TA system TA166624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104784..105210
• Replicon: plasmid pECW601
• Accession: NZ_CP058343
• Organism: Escherichia coli strain W60

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HXP40_RS23365
• Protein ID: WP_001312861.1
• Coordinates: 104784..104942 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104986..105210 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXP40_RS23340	100158..100847	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HXP40_RS23345	101034..101417	-	384	WP_001151524.1	relaxosome protein TraM	-
HXP40_RS23350	101738..102340	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HXP40_RS23355	102637..103458	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HXP40_RS23360	103576..103863	-	288	WP_000107535.1	hypothetical protein	-
HXP40_RS23365	104784..104942	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	104986..105210	-	225	NuclAT_0	-	Antitoxin
-	104986..105210	-	225	NuclAT_0	-	Antitoxin
-	104986..105210	-	225	NuclAT_0	-	Antitoxin
-	104986..105210	-	225	NuclAT_0	-	Antitoxin
HXP40_RS23370	105022..105210	+	189	WP_001299721.1	hypothetical protein	-
HXP40_RS23375	105222..105941	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HXP40_RS23380	105938..106372	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HXP40_RS23385	106427..106624	-	198	Protein_114	hypothetical protein	-
HXP40_RS23390	106652..106885	-	234	WP_000005987.1	DUF905 family protein	-
HXP40_RS23395	106953..107450	-	498	WP_042039995.1	single-stranded DNA-binding protein	-
HXP40_RS23400	107600..109501	-	1902	WP_000936285.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..140410	140410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166624 WP_001312861.1 NZ_CP058343:c104942-104784 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166624 NZ_CP074359:c4136152-4135979 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT166624 NZ_CP058343:c105210-104986 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin