Detailed information of TA system TA166619

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66455..66709
• Replicon: plasmid pECW601
• Accession: NZ_CP058343
• Organism: Escherichia coli strain W60

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HXP40_RS23145
• Protein ID: WP_001351576.1
• Coordinates: 66455..66661 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66648..66709 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXP40_RS23110	62007..62408	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HXP40_RS23115	62341..62598	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HXP40_RS23120	62691..63344	-	654	WP_000616800.1	CPBP family intramembrane metalloprotease	-
HXP40_RS23125	64283..65140	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HXP40_RS23130	65133..65615	-	483	WP_001273588.1	hypothetical protein	-
HXP40_RS23135	65608..65682	-	75	WP_001442103.1	RepA leader peptide Tap	-
HXP40_RS23140	65914..66171	-	258	WP_000083845.1	replication regulatory protein RepA	-
HXP40_RS23145	66455..66661	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66648..66709	+	62	NuclAT_1	-	Antitoxin
-	66648..66709	+	62	NuclAT_1	-	Antitoxin
-	66648..66709	+	62	NuclAT_1	-	Antitoxin
-	66648..66709	+	62	NuclAT_1	-	Antitoxin
HXP40_RS23150	66965..67039	-	75	Protein_67	endonuclease	-
HXP40_RS23155	67285..67497	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HXP40_RS23160	67633..68193	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HXP40_RS23165	68296..69156	-	861	WP_000704514.1	alpha/beta hydrolase	-
HXP40_RS23170	69215..69961	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..140410	140410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T166619 WP_001351576.1 NZ_CP058343:c66661-66455 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T166619 NZ_CP074359:3410303-3410521 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT166619 NZ_CP058343:66648-66709 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin