Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3747019..3747713 Replicon chromosome
Accession NZ_CP058342
Organism Escherichia coli strain W60

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HXP40_RS17820 Protein ID WP_001263489.1
Coordinates 3747019..3747417 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HXP40_RS17825 Protein ID WP_000554758.1
Coordinates 3747420..3747713 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3742607..3742687 - 81 NuclAT_12 - -
- 3742607..3742687 - 81 NuclAT_12 - -
- 3742607..3742687 - 81 NuclAT_12 - -
- 3742607..3742687 - 81 NuclAT_12 - -
HXP40_RS17795 3743283..3743741 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HXP40_RS17800 3744002..3745459 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HXP40_RS17805 3745516..3746037 - 522 Protein_3482 peptide chain release factor H -
HXP40_RS17810 3746033..3746239 - 207 Protein_3483 RtcB family protein -
HXP40_RS17815 3746557..3747009 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HXP40_RS17820 3747019..3747417 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HXP40_RS17825 3747420..3747713 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HXP40_RS17830 3747765..3748820 - 1056 WP_001226164.1 DNA polymerase IV -
HXP40_RS17835 3748891..3749676 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HXP40_RS17840 3749648..3751360 + 1713 Protein_3489 flagellar biosynthesis protein FlhA -
HXP40_RS17845 3751584..3752081 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T166614 WP_001263489.1 NZ_CP058342:c3747417-3747019 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T166614 NZ_CP074359:2258371-2258658 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT166614 WP_000554758.1 NZ_CP058342:c3747713-3747420 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT166614 NZ_CP074359:2258132-2258371 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References