Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1862484..1862986 Replicon chromosome
Accession NZ_CP058342
Organism Escherichia coli strain W60

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HXP40_RS08765 Protein ID WP_000767829.1
Coordinates 1862732..1862986 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HXP40_RS08760 Protein ID WP_001259255.1
Coordinates 1862484..1862735 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXP40_RS08735 1857661..1858728 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HXP40_RS08740 1858728..1859798 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
HXP40_RS08745 1859795..1861099 - 1305 WP_074514295.1 histidinol dehydrogenase -
HXP40_RS08750 1861105..1862004 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HXP40_RS08755 1862150..1862200 - 51 WP_001364200.1 his operon leader peptide -
HXP40_RS08760 1862484..1862735 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HXP40_RS08765 1862732..1862986 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HXP40_RS08770 1863069..1863893 + 825 WP_000754737.1 SDR family oxidoreductase -
HXP40_RS08775 1863939..1864868 + 930 WP_000803366.1 LysR family transcriptional regulator -
HXP40_RS08780 1865083..1865145 + 63 WP_010723108.1 membrane protein YoeI -
HXP40_RS08785 1865135..1866493 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HXP40_RS08790 1866672..1867730 + 1059 WP_087523589.1 transport protein YeeE -
HXP40_RS08795 1867744..1867971 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T166601 WP_000767829.1 NZ_CP058342:1862732-1862986 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T166601 NZ_CP074356:c4670065-4669754 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT166601 WP_001259255.1 NZ_CP058342:1862484-1862735 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT166601 NZ_CP074356:c4669753-4669463 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References