Detailed information of TA system TA166589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 853245..853926
• Replicon: chromosome
• Accession: NZ_CP058333
• Organism: Pseudomonas aeruginosa strain ACR20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HWQ57_RS04045
• Protein ID: WP_015503432.1
• Coordinates: 853245..853610 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HWQ57_RS04050
• Protein ID: WP_003145733.1
• Coordinates: 853603..853926 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ57_RS04015	849241..849675	-	435	WP_003116494.1	RidA family protein	-
HWQ57_RS04020	849896..850849	+	954	WP_003085661.1	LysR family transcriptional regulator	-
HWQ57_RS04025	850822..851706	-	885	WP_003114205.1	LysR family transcriptional regulator	-
HWQ57_RS04030	851807..852232	+	426	WP_003114206.1	ring-cleaving dioxygenase	-
HWQ57_RS04035	852263..852535	-	273	WP_023104842.1	hypothetical protein	-
HWQ57_RS04040	852742..852981	+	240	WP_044060930.1	hypothetical protein	-
HWQ57_RS04045	853245..853610	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ57_RS04050	853603..853926	+	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HWQ57_RS04055	854302..855390	-	1089	WP_003145732.1	DUF3396 domain-containing protein	-
HWQ57_RS04060	855407..856108	-	702	WP_023110332.1	VRR-NUC domain-containing protein	-
HWQ57_RS04065	856105..856623	-	519	WP_031629200.1	PAAR domain-containing protein	-
HWQ57_RS04070	856797..857132	-	336	WP_003140884.1	TM2 domain-containing protein	-
HWQ57_RS04075	857375..858013	-	639	WP_003163192.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	850822..865929	15107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T166589 WP_015503432.1 NZ_CP058333:853245-853610 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T166589 NZ_CP074356:2477521-2477799 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGTGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT166589 WP_003145733.1 NZ_CP058333:853603-853926 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT166589 NZ_CP074356:2477799-2478083 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTTAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACAGGAAAAGCGGCGCTCACTCCGGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA