Detailed information of TA system TA166587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 192777..193372
• Replicon: chromosome
• Accession: NZ_CP058333
• Organism: Pseudomonas aeruginosa strain ACR20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HWQ57_RS00940
• Protein ID: WP_003113526.1
• Coordinates: 192777..193055 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HWQ57_RS00945
• Protein ID: WP_003113527.1
• Coordinates: 193067..193372 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ57_RS00925	188203..189441	+	1239	WP_014603324.1	C69 family dipeptidase	-
HWQ57_RS00930	189503..190150	+	648	WP_003095021.1	carbonate dehydratase	-
HWQ57_RS00935	190220..192448	-	2229	WP_003099265.1	TonB-dependent receptor	-
HWQ57_RS00940	192777..193055	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ57_RS00945	193067..193372	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HWQ57_RS00955	193778..194878	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HWQ57_RS00960	194919..195503	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HWQ57_RS00965	195545..196159	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HWQ57_RS00970	196276..197217	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HWQ57_RS00980	197384..198232	-	849	WP_023110429.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T166587 WP_003113526.1 NZ_CP058333:192777-193055 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T166587 NZ_CP074356:2071018-2071121 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT166587 WP_003113527.1 NZ_CP058333:193067-193372 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT166587 NZ_CP074356:c2071159-2070891 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCATCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCTG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: V6ALY3