Detailed information of TA system TA166581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2747462..2748057
• Replicon: chromosome
• Accession: NZ_CP058332
• Organism: Pseudomonas aeruginosa strain B18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: B18_RS12630
• Protein ID: WP_003113526.1
• Coordinates: 2747462..2747740 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B18_RS12635
• Protein ID: WP_003113527.1
• Coordinates: 2747752..2748057 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B18_RS12615	2742888..2744126	+	1239	WP_014603324.1	C69 family dipeptidase	-
B18_RS12620	2744188..2744835	+	648	WP_003095021.1	carbonate dehydratase	-
B18_RS12625	2744905..2747133	-	2229	WP_003099265.1	TonB-dependent receptor	-
B18_RS12630	2747462..2747740	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B18_RS12635	2747752..2748057	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
B18_RS12645	2748463..2749563	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
B18_RS12650	2749604..2750188	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
B18_RS12655	2750230..2750844	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
B18_RS12660	2750961..2751902	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
B18_RS12670	2752069..2752917	-	849	WP_023110429.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T166581 WP_003113526.1 NZ_CP058332:2747462-2747740 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T166581 NZ_CP074356:277736-277966 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT166581 WP_003113527.1 NZ_CP058332:2747752-2748057 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT166581 NZ_CP074356:277963-278322 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: V6ALY3