Detailed information of TA system TA166579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6016031..6016626
• Replicon: chromosome
• Accession: NZ_CP058331
• Organism: Pseudomonas aeruginosa strain ACR22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HWQ50_RS28035
• Protein ID: WP_003113526.1
• Coordinates: 6016348..6016626 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HWQ50_RS28030
• Protein ID: WP_003113527.1
• Coordinates: 6016031..6016336 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ50_RS27995	6011171..6012019	+	849	WP_023110429.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HWQ50_RS28005	6012186..6013127	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HWQ50_RS28010	6013244..6013858	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HWQ50_RS28015	6013900..6014484	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HWQ50_RS28020	6014525..6015625	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HWQ50_RS28030	6016031..6016336	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HWQ50_RS28035	6016348..6016626	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ50_RS28040	6016955..6019183	+	2229	WP_003099265.1	TonB-dependent receptor	-
HWQ50_RS28045	6019253..6019900	-	648	WP_003095021.1	carbonate dehydratase	-
HWQ50_RS28050	6019962..6021200	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T166579 WP_003113526.1 NZ_CP058331:c6016626-6016348 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T166579 NZ_CP074354:c4169251-4169078 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT166579 WP_003113527.1 NZ_CP058331:c6016336-6016031 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT166579 NZ_CP074354:c4169575-4169279 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: V6ALY3