Detailed information of TA system TA166577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5355479..5356160
• Replicon: chromosome
• Accession: NZ_CP058331
• Organism: Pseudomonas aeruginosa strain ACR22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HWQ50_RS24930
• Protein ID: WP_015503432.1
• Coordinates: 5355795..5356160 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HWQ50_RS24925
• Protein ID: WP_003145733.1
• Coordinates: 5355479..5355802 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ50_RS24900	5351392..5352030	+	639	WP_003163192.1	hypothetical protein	-
HWQ50_RS24905	5352273..5352608	+	336	WP_003140884.1	TM2 domain-containing protein	-
HWQ50_RS24910	5352782..5353300	+	519	WP_031629200.1	PAAR domain-containing protein	-
HWQ50_RS24915	5353297..5353998	+	702	WP_023110332.1	VRR-NUC domain-containing protein	-
HWQ50_RS24920	5354015..5355103	+	1089	WP_003145732.1	DUF3396 domain-containing protein	-
HWQ50_RS24925	5355479..5355802	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HWQ50_RS24930	5355795..5356160	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ50_RS24935	5356424..5356663	-	240	WP_044060930.1	hypothetical protein	-
HWQ50_RS24940	5356870..5357142	+	273	WP_023104842.1	hypothetical protein	-
HWQ50_RS24945	5357173..5357598	-	426	WP_003114206.1	ring-cleaving dioxygenase	-
HWQ50_RS24950	5357699..5358583	+	885	WP_003114205.1	LysR family transcriptional regulator	-
HWQ50_RS24955	5358556..5359509	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HWQ50_RS24960	5359730..5360164	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5343476..5358583	15107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T166577 WP_015503432.1 NZ_CP058331:c5356160-5355795 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T166577 NZ_CP074354:c3935279-3934992 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT166577 WP_003145733.1 NZ_CP058331:c5355802-5355479 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT166577 NZ_CP074354:c3935005-3934703 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTTTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA