Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4963948..4964534 Replicon chromosome
Accession NZ_CP058325
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag GLO21_RS24605 Protein ID WP_002920800.1
Coordinates 4963948..4964316 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag GLO21_RS24610 Protein ID WP_002920802.1
Coordinates 4964313..4964534 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO21_RS24585 (GLO21_024580) 4959451..4960521 - 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
GLO21_RS24590 (GLO21_024585) 4960523..4961368 - 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
GLO21_RS24595 (GLO21_024590) 4961365..4962252 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
GLO21_RS24600 (GLO21_024595) 4962359..4963675 - 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
GLO21_RS24605 (GLO21_024600) 4963948..4964316 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GLO21_RS24610 (GLO21_024605) 4964313..4964534 - 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GLO21_RS24615 (GLO21_024610) 4964698..4965411 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
GLO21_RS24620 (GLO21_024615) 4965413..4966180 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GLO21_RS24625 (GLO21_024620) 4966177..4967454 - 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
GLO21_RS24630 (GLO21_024625) 4967451..4968377 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4958714..4979302 20588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T166569 WP_002920800.1 NZ_CP058325:c4964316-4963948 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T166569 NZ_CP074354:1802916-1803170 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT166569 WP_002920802.1 NZ_CP058325:c4964534-4964313 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT166569 NZ_CP074354:1802668-1802919 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References