Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4937699..4938345 Replicon chromosome
Accession NZ_CP058325
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-1825/18

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag GLO21_RS24495 Protein ID WP_002920560.1
Coordinates 4937998..4938345 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag GLO21_RS24490 Protein ID WP_002920557.1
Coordinates 4937699..4937998 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GLO21_RS24470 (GLO21_024465) 4933905..4934663 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
GLO21_RS24475 (GLO21_024470) 4934713..4935543 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
GLO21_RS24480 (GLO21_024475) 4935594..4935923 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
GLO21_RS24485 (GLO21_024480) 4936128..4937636 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
GLO21_RS24490 (GLO21_024485) 4937699..4937998 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
GLO21_RS24495 (GLO21_024490) 4937998..4938345 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GLO21_RS24500 (GLO21_024495) 4938521..4940968 - 2448 WP_002920561.1 glycogen phosphorylase -
GLO21_RS24505 (GLO21_024500) 4940986..4942419 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T166568 WP_002920560.1 NZ_CP058325:c4938345-4937998 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T166568 NZ_CP074354:1802668-1802919 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT166568 WP_002920557.1 NZ_CP058325:c4937998-4937699 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT166568 NZ_CP074354:1802916-1803170 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References