Detailed information of TA system TA166517

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50480..50906
• Replicon: plasmid pAMPD02
• Accession: NZ_CP058310
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HXS78_RS24115
• Protein ID: WP_001312861.1
• Coordinates: 50480..50638 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	50682..50906 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS24090	45854..46543	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HXS78_RS24095	46730..47113	-	384	WP_001151524.1	relaxosome protein TraM	-
HXS78_RS24100	47434..48036	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HXS78_RS24105	48333..49154	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HXS78_RS24110	49272..49559	-	288	WP_000107535.1	hypothetical protein	-
HXS78_RS24115	50480..50638	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	50682..50906	-	225	NuclAT_0	-	Antitoxin
-	50682..50906	-	225	NuclAT_0	-	Antitoxin
-	50682..50906	-	225	NuclAT_0	-	Antitoxin
-	50682..50906	-	225	NuclAT_0	-	Antitoxin
HXS78_RS24120	50718..50906	+	189	WP_001299721.1	hypothetical protein	-
HXS78_RS24125	50918..51637	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HXS78_RS24130	51634..52068	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HXS78_RS24135	52123..52320	-	198	Protein_62	hypothetical protein	-
HXS78_RS24140	52348..52581	-	234	WP_000005987.1	DUF905 family protein	-
HXS78_RS24145	52649..53188	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
HXS78_RS24150	53214..53420	-	207	WP_000275856.1	hypothetical protein	-
HXS78_RS24155	54559..55530	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..129018	129018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166517 WP_001312861.1 NZ_CP058310:c50638-50480 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166517 NZ_CP074200:c895993-895730 [Halomonas sp. FeN2]
ATGAACGATTTTGAGTTCGATGAAGAGAAAAGTGCAGCCAATCGAGTAAAGCATGGAATCGATTTTCTGGATGCGCAGGC
GCTTTGGAATGATCCTGATTTGCTGGAAATTCAGGCTAAATCCAATGATGAGCCACGTTTTCTTGTAGTAGGACTAATTG
GCGTTAAACACTGGTCTGCTGTCATCACTTATAGAAATGAACGAATTCGACTCATTTCGGTAAGGCGTTCCCGGAAAAGA
GAGGTGGAACTTTATGAAAGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT166517 NZ_CP058310:c50906-50682 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin