Detailed information of TA system TA166512

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12151..12405
• Replicon: plasmid pAMPD02
• Accession: NZ_CP058310
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HXS78_RS23895
• Protein ID: WP_001351576.1
• Coordinates: 12151..12357 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	12344..12405 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS23865	7396..8013	+	618	WP_000521604.1	hypothetical protein	-
HXS78_RS23870	8072..9064	-	993	WP_001579760.1	hypothetical protein	-
HXS78_RS23875	9979..10836	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HXS78_RS23880	10829..11311	-	483	WP_001273588.1	hypothetical protein	-
HXS78_RS23885	11304..11378	-	75	WP_001442103.1	RepA leader peptide Tap	-
HXS78_RS23890	11610..11867	-	258	WP_000083845.1	replication regulatory protein RepA	-
HXS78_RS23895	12151..12357	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	12344..12405	+	62	NuclAT_1	-	Antitoxin
-	12344..12405	+	62	NuclAT_1	-	Antitoxin
-	12344..12405	+	62	NuclAT_1	-	Antitoxin
-	12344..12405	+	62	NuclAT_1	-	Antitoxin
HXS78_RS23900	12661..12735	-	75	Protein_15	endonuclease	-
HXS78_RS23905	12981..13193	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HXS78_RS23910	13329..13889	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HXS78_RS23915	13992..14852	-	861	WP_000704514.1	alpha/beta hydrolase	-
HXS78_RS23920	14911..15657	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..129018	129018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T166512 WP_001351576.1 NZ_CP058310:c12357-12151 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T166512 NZ_CP074186:42687-43169 [Raoultella planticola]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGTAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGTTCACCATGGATTTAAGGCATCACAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT166512 NZ_CP058310:12344-12405 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin