Detailed information of TA system TA166510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4648610..4649212
• Replicon: chromosome
• Accession: NZ_CP058308
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HXS78_RS22225
• Protein ID: WP_000897305.1
• Coordinates: 4648901..4649212 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HXS78_RS22220
• Protein ID: WP_000356397.1
• Coordinates: 4648610..4648900 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS22195	4644535..4645437	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HXS78_RS22200	4645434..4646069	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HXS78_RS22205	4646066..4646995	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HXS78_RS22210	4647325..4647567	-	243	WP_001087409.1	hypothetical protein	-
HXS78_RS22215	4647787..4648005	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
HXS78_RS22220	4648610..4648900	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HXS78_RS22225	4648901..4649212	-	312	WP_000897305.1	hypothetical protein	Toxin
HXS78_RS22230	4649441..4650349	+	909	WP_001162704.1	alpha/beta hydrolase	-
HXS78_RS22235	4650413..4651354	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HXS78_RS22240	4651399..4651836	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HXS78_RS22245	4651833..4652705	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HXS78_RS22250	4652699..4653298	-	600	WP_001295269.1	glucose-1-phosphatase	-
HXS78_RS22255	4653397..4654182	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T166510 WP_000897305.1 NZ_CP058308:c4649212-4648901 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T166510 NZ_CP074182:c5195825-5195337 [Raoultella planticola]
ATGATTTCCGCCCCTGAGCCACTCCGTGCCGAACATGTACTATCTTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTAAAACTACGGGCGATGAAAAATCAGGTCACTGGAGCATCACGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCTGTAGCAATGAATTCAGCTCCCGGACGTTTTCGCCGAAATATGCCCGATCCTATC
CCGGTCGTGATGTTGGGGCGCTTGGCTGTGGATACGTCATTGCATGGCCAAGGTGTTGGCCGTGCTCTGGTACGTGATGC
TGGGCTAAGAGTGATTCAGGTTGCTGATACTATCGGCATTCGTGGGATGCTGGTTCATGCGCTGTCTGATGAGGCACGGG
AATTTTATCTACGGGTGGGGTTTGAACCTTCGCCGATAGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTTGGG
AGTTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT166510 WP_000356397.1 NZ_CP058308:c4648900-4648610 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT166510 NZ_CP074182:c5196115-5195822 [Raoultella planticola]
ATGCCAGCGACGAACAGCGTTTCTGTTAAACGCGAAACACTGAATTTACGCATCAAACCCGCAGAACGGGATCTGATTGA
TCGTGCAGCTAAGGCCAGAGGAAAAAATCGTACCGATTTTGTCCTTGAAGCTGCTCGCGCTGCCGCGGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGTTTGGATCAAGCTCCCTCTCCGAAT
GCCGCGCTGCGTAAAACCATGCAAACACCTGCACCGTGGGAACAGAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7