Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3750992..3751533 Replicon chromosome
Accession NZ_CP058308
Organism Escherichia coli strain AMSCJX04

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HXS78_RS18060 Protein ID WP_000615976.1
Coordinates 3751255..3751533 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HXS78_RS18055 Protein ID WP_000729704.1
Coordinates 3750992..3751252 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS78_RS18030 3746775..3747560 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
HXS78_RS18035 3747505..3749244 + 1740 WP_001296890.1 flagellar biosynthesis protein FlhA -
HXS78_RS18040 3749349..3749627 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HXS78_RS18045 3749620..3749976 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
HXS78_RS18050 3750033..3750806 - 774 WP_000543895.1 C40 family peptidase -
HXS78_RS18055 3750992..3751252 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HXS78_RS18060 3751255..3751533 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HXS78_RS18065 3751689..3752429 + 741 WP_063100007.1 murein L,D-transpeptidase -
HXS78_RS18070 3752400..3753167 - 768 WP_000333380.1 class II glutamine amidotransferase -
HXS78_RS18075 3753373..3753951 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3749620..3762319 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T166507 WP_000615976.1 NZ_CP058308:3751255-3751533 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T166507 NZ_CP074182:c2838434-2837103 [Raoultella planticola]
ATGGCTACTCTCGTGACATGGATGAACAATGAGCGAGTCGGCGAACTTATCAAGCAGGCGAACGGCGCCCATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCTCGCGCCAGGCCGCTCTCTCTGTCGCTCCCGCTGCAGTACGGTAACATCACCG
ACGATGCGGTGTTTAACTATTTTGACAACCTGCTGCCGGACAGCCCGCGGGTGCGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCTTTTGATCTGCTGGCCGAGATCGGACGCGACAGCGTCGGGGCGGTCACGTTACTCCCGCCAGG
GGAAAATGCAACCCGGGGGGCGTTGAGCTGGGAGGCGCTGGATAGCCACGCCCTGGATGCGCTGCTGACGGCCTACCAGT
CGGATATACCGCTCGGGATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATACCGACGGGGACCACGCCGACCACCCATATCATTAAACTGCCTATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTAGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCGCGCCAGCATTATCCAGACCGCCAGCATCAGGGCGCTGGCGGTTGAGCGTTTCGACCGTCGTTGG
GCGCAGGAGAAGACGGTGCTGCTGCGTTTACCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCGATTATGGCTTTTCTGGTTGGATCCAGCGAGGCGCTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCGAAAAACTTCTCGATCTTCCTCCAG
CCGGGCGGCAGCTATCGCTTAACCCCATTCTACGACATTATCTCCGCGTTTCCGCTGCTTGGCGGGAAGGGGCTGCACCT
GAGCGATCTCCGGCTGTCAATGAGCCTGAAGGCCAGTAAAGGGCGCAAAACGGAGATCCAGACGCTCTATCCGCGTCATT
TCCTCGCCACGGCGAAAGAGGTGGGGTTTGCCGAGGAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CACGCGGTTGCTGCGGTAAAGGAAACTTTACCGCATGACTTTTCACCACCGGTGTATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTTACCGTCGGGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT166507 WP_000729704.1 NZ_CP058308:3750992-3751252 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT166507 NZ_CP074182:c2838706-2838434 [Raoultella planticola]
ATGAGCCCTTCACCAGGAATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGACCTGGCTCAGCGTATGGGGATTAAACAGGCTACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCAACGAT
AGCAGTGATAACCCACAGGATCTGGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References