Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1896916..1897418 Replicon chromosome
Accession NZ_CP058308
Organism Escherichia coli strain AMSCJX04

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HXS78_RS09050 Protein ID WP_000767829.1
Coordinates 1897164..1897418 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HXS78_RS09045 Protein ID WP_001259255.1
Coordinates 1896916..1897167 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS78_RS09020 1892094..1893161 - 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HXS78_RS09025 1893161..1894231 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
HXS78_RS09030 1894228..1895532 - 1305 WP_001559366.1 histidinol dehydrogenase -
HXS78_RS09035 1895538..1896437 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HXS78_RS09040 1896583..1896633 - 51 WP_001364200.1 his operon leader peptide -
HXS78_RS09045 1896916..1897167 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HXS78_RS09050 1897164..1897418 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HXS78_RS09055 1897501..1898325 + 825 WP_000754749.1 SDR family oxidoreductase -
HXS78_RS09060 1898371..1899300 + 930 WP_000803351.1 LysR family transcriptional regulator -
HXS78_RS09065 1899515..1899577 + 63 WP_010723108.1 membrane protein YoeI -
HXS78_RS09070 1899567..1900925 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HXS78_RS09075 1901104..1902162 + 1059 WP_023910197.1 transport protein YeeE -
HXS78_RS09080 1902176..1902403 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T166492 WP_000767829.1 NZ_CP058308:1897164-1897418 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T166492 NZ_CP074177:1239214-1239585 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT166492 WP_001259255.1 NZ_CP058308:1896916-1897167 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT166492 NZ_CP074177:1238969-1239211 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGAAAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References