Detailed information of TA system TA166487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 594564..595363
• Replicon: chromosome
• Accession: NZ_CP058308
• Organism: Escherichia coli strain AMSCJX04

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: HXS78_RS02875
• Protein ID: WP_000347266.1
• Coordinates: 594564..595028 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: HXS78_RS02880
• Protein ID: WP_001307405.1
• Coordinates: 595028..595363 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS78_RS02845	589565..589999	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
HXS78_RS02850	590017..590895	-	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HXS78_RS02855	590885..591664	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HXS78_RS02860	591675..592148	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HXS78_RS02865	592171..593451	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HXS78_RS02870	593700..594509	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HXS78_RS02875	594564..595028	-	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HXS78_RS02880	595028..595363	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HXS78_RS02885	595512..597083	-	1572	WP_001273741.1	galactarate dehydratase	-
HXS78_RS02890	597458..598792	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
HXS78_RS02895	598808..599578	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	594564..606351	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T166487 WP_000347266.1 NZ_CP058308:c595028-594564 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T166487 NZ_CP074177:57116-57445 [Enterobacter asburiae]
ATGCAAACCTTATCATCACACCCGACGCAGGCGGCTCAGCCCTGCCTGTCACCCATTGAAATCTGGCAGTGTCTGCTAAC
TCATCTGTTGTCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTAATGAAACCACCATACGAGAACATATTG
ATGCAGGAATATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGGCTTGTCCGTATCGATCGGAAAGGATTT
TCGTGGCAAGAGCAGACCCCGTATCTTTCCGTAGTGGATATTTTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
CGTGAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT166487 WP_001307405.1 NZ_CP058308:c595363-595028 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT166487 NZ_CP074177:56712-57077 [Enterobacter asburiae]
ATGAACAATCACTCCGAATCCGGTACTAAGCCGGAGAGTCCCGCCTGCCAGCGGTGGGGACTTAAACGTGCTATTACCCC
CTGTTTCGGGGCTCGTCTGGTACAGGAAGGCAATCGGGTACATTTTCTCGCTGACCGTGCCGGGTTTAACGGTGCTTTCA
GTGACAACGATGCATTGCGCCTCGACCAGGCCTTTCCGTTGATGCTCAAACAACTGGAGTTGATGCTGATTAGCAGCGAG
CTCAATCCCCGCTATCAGCACTGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTACATTGCTATCTATCCTGAGCAGACTGAGCCGCATTAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7