Detailed information of TA system TA166476

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100184..100438
• Replicon: plasmid pAH65-3
• Accession: NZ_CP058305
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HUU85_RS24125
• Protein ID: WP_001351576.1
• Coordinates: 100232..100438 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	100184..100245 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS24090	95607..96296	+	690	Protein_93	DUF87 domain-containing protein	-
HUU85_RS24095	96295..96912	+	618	Protein_94	conjugative transfer relaxase/helicase TraI	-
HUU85_RS24100	96932..97678	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
HUU85_RS24105	97737..98597	+	861	WP_000704513.1	alpha/beta hydrolase	-
HUU85_RS24110	98700..99260	+	561	WP_176256657.1	fertility inhibition protein FinO	-
HUU85_RS24115	99396..99608	+	213	WP_001299730.1	ANR family transcriptional regulator	-
HUU85_RS24120	99854..99928	+	75	Protein_99	endonuclease	-
-	100184..100245	-	62	NuclAT_1	-	Antitoxin
-	100184..100245	-	62	NuclAT_1	-	Antitoxin
-	100184..100245	-	62	NuclAT_1	-	Antitoxin
-	100184..100245	-	62	NuclAT_1	-	Antitoxin
HUU85_RS24125	100232..100438	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUU85_RS24130	100722..100979	+	258	WP_000083845.1	replication regulatory protein RepA	-
HUU85_RS24135	101211..101285	+	75	WP_001442103.1	RepA leader peptide Tap	-
HUU85_RS24140	101278..101760	+	483	WP_001273588.1	hypothetical protein	-
HUU85_RS24145	101753..102610	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HUU85_RS24150	103550..104203	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUU85_RS24155	104296..104553	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUU85_RS24160	104486..104887	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / sitABCD / floR / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A)	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..139513	139513
	-	flank	IS/Tn	-	-	94834..95490	656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T166476 WP_001351576.1 NZ_CP058305:100232-100438 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T166476 NZ_CP074170:c4126788-4126378 [Enterobacter sp. JBIWA003]
GTGGTCCTGTGGCAATCTGATCTTCGCGTTTCGTGGCGCTCGCAGTGGATGTCTTTGTTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTCTCGTTTGTCGTGTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGCTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT166476 NZ_CP058305:c100245-100184 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin