Detailed information of TA system TA166473

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89672..89941
• Replicon: plasmid pAH65-3
• Accession: NZ_CP058305
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUU85_RS24050
• Protein ID: WP_001312861.1
• Coordinates: 89783..89941 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89672..89737 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS24020	85224..87125	+	1902	WP_058914895.1	group II intron reverse transcriptase/maturase	-
HUU85_RS24025	87275..87772	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
HUU85_RS24030	87840..88073	+	234	WP_000005990.1	DUF905 family protein	-
HUU85_RS24035	88101..88298	+	198	Protein_82	hypothetical protein	-
HUU85_RS24040	88353..88787	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HUU85_RS24045	88784..89533	+	750	WP_072648274.1	plasmid SOS inhibition protein A	-
-	89515..89739	+	225	NuclAT_0	-	-
-	89515..89739	+	225	NuclAT_0	-	-
-	89515..89739	+	225	NuclAT_0	-	-
-	89515..89739	+	225	NuclAT_0	-	-
-	89672..89737	-	66	-	-	Antitoxin
HUU85_RS24050	89783..89941	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUU85_RS24055	90862..91149	+	288	WP_000107535.1	hypothetical protein	-
HUU85_RS24060	91267..92088	+	822	WP_001234426.1	DUF945 domain-containing protein	-
HUU85_RS24065	92385..92987	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HUU85_RS24070	93308..93691	+	384	WP_001151524.1	relaxosome protein TraM	-
HUU85_RS24075	93878..94567	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HUU85_RS24080	94666..94821	+	156	Protein_91	TraY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / sitABCD / floR / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A)	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..139513	139513
	-	flank	IS/Tn	-	-	94834..95490	656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166473 WP_001312861.1 NZ_CP058305:89783-89941 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166473 NZ_CP074170:c1643822-1643349 [Enterobacter sp. JBIWA003]
ATGATTTTTTATCGTCTGGTGACAGGCCGCTATGCCAGCGAAGCGTGGAGTGGAAGTGGCGCAAATCAGTATGGCGGACG
GTGGAATCATAAAGGCCATCCAGCTGTGTATGTCTCCACCTCTATCTCGCTGGCCTCACTGGAGATTTTGGTACATGTCA
GAAAAGATACCGTGCTGAATCAGTACCAGCTTTTCAGCATTGATATCCCGGATGACCAGATAGATTACCTGGATAAAGCG
TGGCTCCCTGAGGACTGGCAGGAAAATCCGGCGCCGGTATCGACAATGGATCTGGGAACCGGTTGGTTGCAGGCTAACAG
TGCCCTCGCGCTCATCTTACCCTCCAGTATCATCCCGTATGAAAACAATGCCATCCTCAACCCCTTACATCCGGCGTTCC
ACCCGGCGCTAAATTCAGTTCAGCAACTCCCCTTTGTTTTTGATACCCGCCTGACAGAAAAAACCGCACCCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT166473 NZ_CP058305:c89737-89672 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin