Detailed information of TA system TA166467

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39433..39697
• Replicon: plasmid pAH65-2
• Accession: NZ_CP058304
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HUU85_RS23400
• Protein ID: WP_001331364.1
• Coordinates: 39545..39697 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	39433..39490 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS23385	35771..36841	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HUU85_RS23390	36860..38068	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
HUU85_RS23395	38286..39272	-	987	WP_001257838.1	hypothetical protein	-
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
HUU85_RS23400	39545..39697	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HUU85_RS23405	39769..40020	-	252	WP_001291964.1	hypothetical protein	-
HUU85_RS23410	40679..40855	-	177	WP_001054901.1	hypothetical protein	-
HUU85_RS23415	41187..41396	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
HUU85_RS23420	41468..42130	-	663	WP_060527638.1	plasmid IncI1-type surface exclusion protein ExcA	-
HUU85_RS23425	42201..44369	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84033	84033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T166467 WP_001331364.1 NZ_CP058304:39545-39697 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T166467 NZ_CP074168:c4847612-4847337 [Serratia ureilytica]
ATGGAGTTAAAATGGACGAGTAAGGCGCTACCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGACG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Nucleotide

• Length: 58 bp

>AT166467 NZ_CP058304:c39490-39433 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin