Detailed information of TA system TA166459

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4530094..4530465
• Replicon: chromosome
• Accession: NZ_CP058302
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HUU85_RS21650
• Protein ID: WP_001317028.1
• Coordinates: 4530271..4530465 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4530094..4530272 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS21625	4526049..4527422	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
HUU85_RS21630	4527551..4528486	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HUU85_RS21635	4528538..4529773	-	1236	WP_000040852.1	site-specific integrase	-
HUU85_RS21640	4529775..4529990	-	216	WP_000079604.1	excisionase XisR	-
HUU85_RS21645	4530069..4530278	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	4530094..4530272	+	179	NuclAT_0	-	Antitoxin
-	4530094..4530272	+	179	NuclAT_0	-	Antitoxin
-	4530094..4530272	+	179	NuclAT_0	-	Antitoxin
-	4530094..4530272	+	179	NuclAT_0	-	Antitoxin
HUU85_RS21650	4530271..4530465	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HUU85_RS21655	4530522..4531331	-	810	WP_000166319.1	recombination protein RecT	-
HUU85_RS21660	4531324..4533924	-	2601	WP_044502199.1	exodeoxyribonuclease VIII	-
HUU85_RS21665	4534026..4534301	-	276	WP_000632297.1	protein RacC	-
HUU85_RS21670	4534376..4534546	-	171	WP_001352098.1	conserved protein, Rac prophage	-
HUU85_RS21675	4534546..4534767	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4528538..4552215	23677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T166459 WP_001317028.1 NZ_CP058302:c4530465-4530271 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T166459 NZ_CP074168:505067-505321 [Serratia ureilytica]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT166459 NZ_CP058302:4530094-4530272 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin