Detailed information of TA system TA166455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3318608..3319302
• Replicon: chromosome
• Accession: NZ_CP058302
• Organism: Escherichia coli strain AH65

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HUU85_RS15735
• Protein ID: WP_001263493.1
• Coordinates: 3318904..3319302 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HUU85_RS15730
• Protein ID: WP_000554757.1
• Coordinates: 3318608..3318901 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU85_RS15710	3314170..3314667	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
HUU85_RS15715	3314961..3316700	-	1740	WP_001325255.1	flagellar biosynthesis protein FlhA	-
HUU85_RS15720	3316645..3317430	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HUU85_RS15725	3317501..3318556	+	1056	WP_001226164.1	DNA polymerase IV	-
HUU85_RS15730	3318608..3318901	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUU85_RS15735	3318904..3319302	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUU85_RS15740	3319312..3319764	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HUU85_RS15745	3319998..3320264	+	267	WP_001321003.1	hypothetical protein	-
HUU85_RS15750	3320212..3320733	+	522	Protein_3057	peptide chain release factor H	-
HUU85_RS15755	3320790..3322247	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HUU85_RS15760	3322508..3322966	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HUU85_RS15765	3323058..3324302	+	1245	WP_000189541.1	esterase FrsA	-
-	3323562..3323642	+	81	NuclAT_9	-	-
-	3323562..3323642	+	81	NuclAT_9	-	-
-	3323562..3323642	+	81	NuclAT_9	-	-
-	3323562..3323642	+	81	NuclAT_9	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T166455 WP_001263493.1 NZ_CP058302:3318904-3319302 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T166455 NZ_CP074161:c81449-80967 [Enterobacter sp. JBIWA005]
ATGGGAATAAGCGCACCAGAACCGCTTAACAGCGGACATAACATTGCTGATTTTTGCTGCACCGAACCGGGGCTGAATGA
ATGGTTAAAAAAGAAGGCACATAAAAATCACAGCACCGGCATTTCCCGCGTGTATGTTATTTGTGCGGAAAACAGTCATC
GGGTTATCGGATATTACTGCTTATCGACCGGCAGTGTTCAGCGTAATACCGCTCCGGGCGCTTACAGACGCAACGCGCCG
GAATCACTCCCCGTCATTGTTCTCGGCAGACTTGCCGTCGACCAGGCTTATTCCGGCCAGGGGCTTGGCGTTGCCTTGCT
TAAGGACGCGGTATACCGGACTGAACACATCGCACTACAGGTCGGCGTTAAAGCACTGGTTGTTCATGCACTGAGTGAAG
AGGTCAGTAGTTTCTATACCCGATTCGGCTTTGACGCTTCAGTCGTTCACCCGTTAACGCTGCTGTATCCGGTTAAACTT
TAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT166455 WP_000554757.1 NZ_CP058302:3318608-3318901 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT166455 NZ_CP074161:c81718-81440 [Enterobacter sp. JBIWA005]
ATGAAGACCGAAGCCAGAGAAGCCCCGATTAACATCCGGGCAAGAACTTCACAGCGTGATCTTATCGATATGGCGGCAAG
CCTTGTCTCAAAATCACGAACTGACTTTATGCTGGACGCTGCCTGCCGAGAGGCTCAGGACATTCTCCTGGATCAGCGTT
TGTTTATTCTCGATAACGCGCAATATGAATCGTTTATTGCCGAACTGGATGCACCTGTCACAGCGGAACGCCAGGCCCGC
ATTGACGATCTGATGGGCCACAAATCCCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6