Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3312494..3313035 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUU85_RS15695 Protein ID WP_000615976.1
Coordinates 3312494..3312772 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUU85_RS15700 Protein ID WP_000729704.1
Coordinates 3312775..3313035 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS15680 3310076..3310654 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HUU85_RS15685 3310860..3311627 + 768 WP_000333380.1 class II glutamine amidotransferase -
HUU85_RS15690 3311598..3312338 - 741 WP_001225679.1 murein L,D-transpeptidase -
HUU85_RS15695 3312494..3312772 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUU85_RS15700 3312775..3313035 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUU85_RS15705 3313221..3313994 + 774 WP_015675129.1 C40 family peptidase -
HUU85_RS15710 3314170..3314667 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HUU85_RS15715 3314961..3316700 - 1740 WP_001325255.1 flagellar biosynthesis protein FlhA -
HUU85_RS15720 3316645..3317430 + 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T166454 WP_000615976.1 NZ_CP058302:c3312772-3312494 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T166454 NZ_CP074161:c63114-62827 [Enterobacter sp. JBIWA005]
ATGACTTATACAGTCAAATTCAGGGACGATGCATTGAAAGAATGGCAAAAGCTGGACAAGCCCATTCAGCAGCAGTTTGC
AAAAAAGCTGAAGAAGTGCAGTGAAAATCCGCATATCCCTTCAGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATCA
AACTTCGAGCGTCTGGCTTTCGTCTGGTCTATCAGGTGATTGACGATCAATTAATTATCGCTGTTGTAGCTGTAGGAAAA
CGTGAACGCAGTGAAGTTTATAATCTTGCCAGTGAACGAATGAGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT166454 WP_000729704.1 NZ_CP058302:c3313035-3312775 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT166454 NZ_CP074161:c63362-63111 [Enterobacter sp. JBIWA005]
ATGCCCAACATCATTCTCAGTGACACAAGTGCCAGTGTCAGCGAACTCAAGAAAAACCCTATGGCAACGGTCAGCGCTGG
TGATGGCTACCCGGTTGCTATTCTGAACCGCAACCAGCCAGCTTTTTACTGTGTTCCGGCAGAGCTGTACGAGAAGATGC
TCGATGCCCTCGACGATCAGGAACTGGTTAAGCTGGTCGCAGAACGCAGCAGCCAGACGCTTCACGACGTGGATCTGGAT
AGCTACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References