Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1594149..1594876 Replicon chromosome
Accession NZ_CP058302
Organism Escherichia coli strain AH65

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUU85_RS07640 Protein ID WP_000550189.1
Coordinates 1594562..1594876 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUU85_RS07635 Protein ID WP_000560266.1
Coordinates 1594149..1594565 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU85_RS07625 1589309..1591660 + 2352 WP_000695495.1 alpha-glucosidase -
HUU85_RS07630 1592086..1594104 + 2019 WP_000121453.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUU85_RS07635 1594149..1594565 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUU85_RS07640 1594562..1594876 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUU85_RS07645 1595160..1596296 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUU85_RS07650 1596381..1596884 + 504 WP_001300832.1 M48 family metallopeptidase -
HUU85_RS07655 1596961..1597653 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HUU85_RS07660 1597732..1598718 + 987 WP_176256439.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T166449 WP_000550189.1 NZ_CP058302:c1594876-1594562 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T166449 NZ_CP074160:c122532-122122 [Enterobacter sp. JBIWA005]
GTGAACAAGACCTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAACAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTGGTCTGAGATTTCATTCGCCGCCCGGGCTG
CAGGGCCGGTCACGCAGCAGCTGGCTGAAGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCCGCGGTG
GATGCGACCACGGAGGTAAAGGTGGCGCTGCGCCTCGCCGGGACGCCGCCAGGCCCGAACGACACGGCGATAGCCGGGCA
CGCCATCGCCGCCGGCGCGGTGCTGGTGACGAGGATCGGGGGAGAGTTTGAACGGGTACCGGGGTTAATGCTTGAAGACT
GGGTGAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT166449 WP_000560266.1 NZ_CP058302:c1594565-1594149 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT166449 NZ_CP074160:c122759-122529 [Enterobacter sp. JBIWA005]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGCGTCAG
CGAGCTGGTGATTGTCCGGGAAGGAGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References