Detailed information of TA system TA166404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 77067..77655
• Replicon: chromosome
• Accession: NZ_CP058252
• Organism: Helicobacter pylori strain NP05-124

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: J0DAB9
• Locus tag: HWU18_RS00410
• Protein ID: WP_000921927.1
• Coordinates: 77067..77348 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: HWU18_RS00415
• Protein ID: WP_108298292.1
• Coordinates: 77341..77655 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWU18_RS00375	72268..72531	+	264	WP_025276164.1	hypothetical protein	-
HWU18_RS00380	72528..72826	+	299	Protein_68	hypothetical protein	-
HWU18_RS00385	72826..73767	+	942	WP_237004392.1	ATPase, T2SS/T4P/T4SS family	-
HWU18_RS00390	73764..74219	+	456	WP_025276161.1	hypothetical protein	-
HWU18_RS00395	74216..74506	+	291	WP_000159826.1	hypothetical protein	-
HWU18_RS00400	74507..75064	+	558	WP_025277016.1	hypothetical protein	-
HWU18_RS00405	75065..76792	+	1728	WP_237004393.1	type IV secretory system conjugative DNA transfer family protein	-
HWU18_RS00410	77067..77348	-	282	WP_000921927.1	YafQ family addiction module toxin	Toxin
HWU18_RS00415	77341..77655	-	315	WP_108298292.1	hypothetical protein	Antitoxin
HWU18_RS00420	77958..79844	+	1887	WP_237004394.1	relaxase	-
HWU18_RS00425	79869..81047	-	1179	WP_237004395.1	hypothetical protein	-
HWU18_RS00430	81079..81363	-	285	WP_180437205.1	hypothetical protein	-
HWU18_RS00435	81445..82101	-	657	WP_237004396.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10931.95 Da
• Isoelectric Point: 10.1597

>T166404 WP_000921927.1 NZ_CP058252:c77348-77067 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSIVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T166404 NZ_CP074147:c720749-720426 [Serratia sp. JSRIV001]
ATGGAATACTTTGAGTTCATCGAAACCCCAGTTTTTAGCAGGCAACGCGAAACCCTATTAGACGATGATAGCTTTCGTGA
ACTACAAACCCACCTGTTAAAATATCACTCAGACGGCGATACTATAAGTAAAACAGGCGGTTGTAAAAAAATCCGATGGA
ACCGTCCAGGCATGGGGAAACAAGGTGGTGTAAGAGTTATCTATTACGTTATCACCCGCAGCGGCAAGCTTTACCTACTG
ATTGTTTATCCTAAAAACGCCAAAGACGACTTAACCGAATCCGAGAAAGCGATACTTAAAGCGTTAACGGATAAACTGAA
TTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12178.66 Da
• Isoelectric Point: 5.6761

>AT166404 WP_108298292.1 NZ_CP058252:c77655-77341 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTPSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT166404 NZ_CP074147:c720383-720093 [Serratia sp. JSRIV001]
GTGGACAAAGAACTTTTTAACGATCTGGTCGAAAGCATGGAACAGATGGTCGCGATTGAAAAAGGCGAAATGCAGCCTGC
TCGTGTCCATCGCCATCATATCCCCAACGTGAAATCACTACGCGAAGCCAGCGGCATGAAGCAGGAAGAGTTTGCGCAGG
CGGTTGGAGTTAGTCCTTCCCTAGTGCAAAGTTGGGAACAAAATCGCCGAATACCCTCCGGCTCTTCACTCAAAATCTTG
CTGATGATTGAGCGCCACCCCACAATTGTTAACGATCTACTCACCATCTAA

Structures

Toxin

• AlphaFold DB: A0A060CQM7