Detailed information of TA system TA166403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1436511..1437099
• Replicon: chromosome
• Accession: NZ_CP058251
• Organism: Helicobacter pylori strain UBN18

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K2LF18
• Locus tag: HWU19_RS06840
• Protein ID: WP_000921926.1
• Coordinates: 1436818..1437099 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: K2KPE8
• Locus tag: HWU19_RS06835
• Protein ID: WP_000065300.1
• Coordinates: 1436511..1436825 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWU19_RS06810 (1431832)	1431832..1432500	-	669	Protein_1324	conjugal transfer protein TrbL	-
HWU19_RS06815 (1432497)	1432497..1432670	+	174	Protein_1325	ParA family protein	-
HWU19_RS06820 (1432749)	1432749..1433033	+	285	WP_000394639.1	hypothetical protein	-
HWU19_RS06825 (1433077)	1433077..1434240	+	1164	WP_237016397.1	hypothetical protein	-
HWU19_RS06830 (1434265)	1434265..1436175	-	1911	Protein_1328	relaxase/mobilization nuclease domain-containing protein	-
HWU19_RS06835 (1436511)	1436511..1436825	+	315	WP_000065300.1	hypothetical protein	Antitoxin
HWU19_RS06840 (1436818)	1436818..1437099	+	282	WP_000921926.1	YafQ family addiction module toxin	Toxin
HWU19_RS06845 (1437374)	1437374..1439101	-	1728	WP_237016398.1	type IV secretory system conjugative DNA transfer family protein	-
HWU19_RS06850 (1439102)	1439102..1439529	-	428	Protein_1332	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	babB/hopT / pflA / babA/hopS / babB/hopT	1241131..1448740	207609

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10932.89 Da
• Isoelectric Point: 9.8257

>T166403 WP_000921926.1 NZ_CP058251:1436818-1437099 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSIVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T166403 NZ_CP074147:672787-673122 [Serratia sp. JSRIV001]
ATGATGATTATCAACACAGCCACACCGATTACCGCGAATGCCCGTCAAGCTTTGGCAGAGTATCAGCAACTACTTGCCTG
GTTGCTGGCTCACTGTTACGGCCTGGAGCTTAACGATACGCCCTATCACGACGATCAGGCCATAGCACAACAGATTGAGC
ATGGCGTCACGGTACGGGAAACCGTCAATGAACTGGTGGAGAAGTTCGACCTGGTACGCATTGATCGCCCCGGATTCAGT
CTGATGGCACAAGACCCGACCCTGAACGGTGGCGATATGTTACGTGCCCGCAGTGCGCTGGGCTTGCGCAGCCCGGTCAT
TAGACGCCTCACCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12194.65 Da
• Isoelectric Point: 5.6761

>AT166403 WP_000065300.1 NZ_CP058251:1436511-1436825 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTPSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT166403 NZ_CP074147:673208-673576 [Serratia sp. JSRIV001]
ATGAAGGAAACACAATCCAATATCACCAGCAACGACAGTACTCCGTCGCCTGTCTGGGGCCTACAATCTGCTATCACACC
ACGCCTGGGAGCCCGGTTGGTCCAGCAGGGTAATCATCTGCATTTTCTGGCTGACCGTGCCGGTTTTGTTGGCACATTTG
ATCCTGATGCGCTTAAAACGCTGGATGCCGCTTTCCCTGCGCTGATTGAACAGTTGGAAGCCTGTCTGCGGTCTGGTGAA
CTCGATCCACGCCGTCAGCATTGCGTCACCGTCCAGCACGCCGGTTTTAGCTGTGAAGCGGATTCGCTAGGTAGTTTTGG
GTATGTCTATATCGCTGTTTATCAGACAACACCGCCCAACCCGGTATAA

Structures

Toxin

• AlphaFold DB: K2LF18

Antitoxin

• AlphaFold DB: K2KPE8