Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2288380..2289299 Replicon chromosome
Accession NZ_CP058236
Organism Mycobacterium tuberculosis strain 060827

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag HWS75_RS10755 Protein ID WP_003900449.1
Coordinates 2288694..2289299 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag HWS75_RS10750 Protein ID WP_003410124.1
Coordinates 2288380..2288685 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWS75_RS10715 2283643..2284218 + 576 WP_003410100.1 hypothetical protein -
HWS75_RS10720 2284215..2285255 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
HWS75_RS10725 2285497..2286216 + 720 WP_003410108.1 DUF433 domain-containing protein -
HWS75_RS10730 2286206..2286385 + 180 Protein_2120 hypothetical protein -
HWS75_RS10740 2287741..2287980 + 240 Protein_2122 hypothetical protein -
HWS75_RS10745 2287996..2288295 - 300 WP_003410120.1 hypothetical protein -
HWS75_RS10750 2288380..2288685 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
HWS75_RS10755 2288694..2289299 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HWS75_RS10760 2289324..2289683 - 360 WP_003410131.1 hypothetical protein -
HWS75_RS10765 2289843..2290496 - 654 WP_014324107.1 hypothetical protein -
HWS75_RS10770 2290472..2290708 - 237 WP_003410133.1 hypothetical protein -
HWS75_RS10775 2290915..2291811 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T166356 WP_003900449.1 NZ_CP058236:c2289299-2288694 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T166356 NZ_CP074140:169128-169460 [Serratia sp. JSRIV004]
ATGAAGGCTATCGATAAAGTACTCCATACCGCCCCCAAAAAGCCTGCCTTGATCGAATACCAACAATTGCTCGCTTGGTT
ACTGGACCACTGCTACGGACTCACGCTCAACGACACGCCATACCACGACCCACTGGCTATTGCCCAACAGATTGAGTATG
GGGTGACCGTACGTGAAACCATCAACGAACTGGTTCAAAAGTTCGACCTGGTACGCGTTGACCGTCCTGGATTCAGTTTG
TTGGCACAAGATCCGACCCTGAGCGGCAGCGATATGTTGCGGGTTCGCAGCGCTCTGGGGCTGCGAACCCCCATTAATTC
CCGCCTGGTTTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT166356 WP_003410124.1 NZ_CP058236:c2288685-2288380 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT166356 NZ_CP074140:169537-169911 [Serratia sp. JSRIV004]
ATGGTGAAAATCCCCGATCCAAAAGCCCCGAACAACATTCCTGACCCTACACCATTCTGGGGCCTGCAACCTGACATCAC
ACCGCGCTTGGGAGCTCGGCTGGTACAGCAGGGGGCTCATCTGCATTTTCTGAGTGACCGAGCTGGCTGTGTGGGTGCTT
TTTCCCCTGAGGCTATCCAGGCTCTTGAGCTGGCATTTCCTGTGTTGGTTGAAACGCTGGAGACACTATTACGGTCTGGT
GAGCTCGGTCCACGCCGCCAACATTGTGTTGCTGTTCAGCATGCGGGTTTTACCTGTGAAGCCGATACGTTGGGCAGTTT
TGGTTATGTGTATATCGCCGTCTATCAAACGGCACGGCCACCCGTTGCAGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References