Detailed information of TA system TA166313

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48379..48643
• Replicon: plasmid pKPCZA02_4
• Accession: NZ_CP058230
• Organism: Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HWQ20_RS27940
• Protein ID: WP_001331364.1
• Coordinates: 48491..48643 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	48379..48441 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ20_RS27925 (43618)	43618..45909	-	2292	WP_001289276.1	F-type conjugative transfer protein TrbC	-
HWQ20_RS27930 (45902)	45902..46972	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HWQ20_RS27935 (46991)	46991..48199	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (48379)	48379..48441	-	63	NuclAT_0	-	Antitoxin
- (48379)	48379..48441	-	63	NuclAT_0	-	Antitoxin
- (48379)	48379..48441	-	63	NuclAT_0	-	Antitoxin
- (48379)	48379..48441	-	63	NuclAT_0	-	Antitoxin
HWQ20_RS27940 (48491)	48491..48643	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HWQ20_RS27945 (48715)	48715..48966	-	252	WP_001291964.1	hypothetical protein	-
HWQ20_RS28445 (49328)	49328..49560	+	233	Protein_61	hypothetical protein	-
HWQ20_RS27950 (49625)	49625..49801	-	177	WP_001054897.1	hypothetical protein	-
HWQ20_RS27955 (50193)	50193..50402	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HWQ20_RS27960 (50474)	50474..51124	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
HWQ20_RS27965 (51198)	51198..53366	-	2169	WP_000698357.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-33	-	1..93354	93354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T166313 WP_001331364.1 NZ_CP058230:48491-48643 [Klebsiella pneumoniae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T166313 NZ_CP074120:1996977-1997255 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT166313 NZ_CP058230:c48441-48379 [Klebsiella pneumoniae]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin