Detailed information of TA system TA166308

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107595..107864
• Replicon: plasmid pKPCZA02_2
• Accession: NZ_CP058228
• Organism: Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HWQ20_RS27300
• Protein ID: WP_001372321.1
• Coordinates: 107739..107864 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107595..107660 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ20_RS27275	103305..103832	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HWQ20_RS27280	103890..104123	+	234	WP_000006003.1	DUF905 family protein	-
HWQ20_RS27285	104184..106207	+	2024	Protein_142	ParB/RepB/Spo0J family partition protein	-
HWQ20_RS27290	106276..106710	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HWQ20_RS27295	106707..107426	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	107438..107662	+	225	NuclAT_0	-	-
-	107438..107662	+	225	NuclAT_0	-	-
-	107438..107662	+	225	NuclAT_0	-	-
-	107438..107662	+	225	NuclAT_0	-	-
-	107595..107660	-	66	-	-	Antitoxin
HWQ20_RS28420	107648..107797	+	150	Protein_145	plasmid maintenance protein Mok	-
HWQ20_RS27300	107739..107864	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HWQ20_RS27305	108183..108479	-	297	Protein_147	hypothetical protein	-
HWQ20_RS27310	108779..109075	+	297	WP_001272251.1	hypothetical protein	-
HWQ20_RS27315	109186..110007	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HWQ20_RS27320	110304..110951	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HWQ20_RS27325	111228..111611	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HWQ20_RS27330	111802..112488	+	687	WP_015059009.1	PAS domain-containing protein	-
HWQ20_RS27335	112582..112809	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..149200	149200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T166308 WP_001372321.1 NZ_CP058228:107739-107864 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T166308 NZ_CP074120:c749171-748995 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT166308 NZ_CP058228:c107660-107595 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin