Detailed information of TA system TA166305

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69355..69608
• Replicon: plasmid pKPCZA02_2
• Accession: NZ_CP058228
• Organism: Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HWQ20_RS27050
• Protein ID: WP_001312851.1
• Coordinates: 69459..69608 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	69355..69414 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ20_RS27010 (65015)	65015..65080	-	66	Protein_86	helix-turn-helix domain-containing protein	-
HWQ20_RS27015 (65133)	65133..65837	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HWQ20_RS27020 (65862)	65862..66062	+	201	WP_072354025.1	hypothetical protein	-
HWQ20_RS27025 (66082)	66082..66828	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HWQ20_RS27030 (66883)	66883..67443	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HWQ20_RS27035 (67575)	67575..67775	+	201	WP_015059022.1	hypothetical protein	-
HWQ20_RS27040 (68161)	68161..68760	+	600	WP_032083981.1	PIN domain-containing protein	-
HWQ20_RS27045 (68822)	68822..69154	+	333	WP_152916585.1	hypothetical protein	-
- (69355)	69355..69414	-	60	NuclAT_1	-	Antitoxin
- (69355)	69355..69414	-	60	NuclAT_1	-	Antitoxin
- (69355)	69355..69414	-	60	NuclAT_1	-	Antitoxin
- (69355)	69355..69414	-	60	NuclAT_1	-	Antitoxin
HWQ20_RS27050 (69459)	69459..69608	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HWQ20_RS27055 (69892)	69892..70140	+	249	WP_000083837.1	replication regulatory protein RepA	-
HWQ20_RS28545 (70385)	70385..70459	+	75	WP_001375168.1	RepA leader peptide Tap	-
HWQ20_RS27065 (70452)	70452..71309	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
HWQ20_RS27070 (72248)	72248..72901	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HWQ20_RS27075 (72994)	72994..73251	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HWQ20_RS27080 (73184)	73184..73585	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HWQ20_RS27085 (73834)	73834..74249	+	416	Protein_101	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..149200	149200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T166305 WP_001312851.1 NZ_CP058228:69459-69608 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T166305 NZ_CP074120:c117537-117160 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT166305 NZ_CP058228:c69414-69355 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin