Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3636403..3636965 Replicon chromosome
Accession NZ_CP058223
Organism Escherichia coli strain 550

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HWQ66_RS17130 Protein ID WP_000605676.1
Coordinates 3636403..3636681 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HWQ66_RS17135 Protein ID WP_000781370.1
Coordinates 3636681..3636965 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ66_RS17105 3631989..3632420 - 432 WP_000152305.1 peroxiredoxin OsmC -
HWQ66_RS17110 3632765..3632980 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HWQ66_RS17115 3633082..3633219 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HWQ66_RS17120 3633376..3635073 + 1698 WP_000433464.1 malate dehydrogenase -
HWQ66_RS17125 3635207..3636217 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HWQ66_RS17130 3636403..3636681 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HWQ66_RS17135 3636681..3636965 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HWQ66_RS17140 3637193..3637846 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HWQ66_RS17145 3637839..3638723 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
HWQ66_RS17150 3638736..3641783 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T166277 WP_000605676.1 NZ_CP058223:3636403-3636681 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T166277 NZ_CP074105:415292-415633 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT166277 WP_000781370.1 NZ_CP058223:3636681-3636965 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT166277 NZ_CP074105:415062-415292 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References