Detailed information of TA system TA166256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5020143..5020745
• Replicon: chromosome
• Accession: NZ_CP058222
• Organism: Escherichia coli strain CFT073

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HW370_RS24285
• Protein ID: WP_000897302.1
• Coordinates: 5020434..5020745 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW370_RS24280
• Protein ID: WP_000356397.1
• Coordinates: 5020143..5020433 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW370_RS24255 (5016216)	5016216..5017118	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HW370_RS24260 (5017115)	5017115..5017750	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HW370_RS24265 (5017747)	5017747..5018676	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HW370_RS24270 (5018892)	5018892..5019110	-	219	WP_001314326.1	CopG family transcriptional regulator	-
HW370_RS24275 (5019506)	5019506..5019784	-	279	WP_001296612.1	hypothetical protein	-
HW370_RS24280 (5020143)	5020143..5020433	-	291	WP_000356397.1	NadS family protein	Antitoxin
HW370_RS24285 (5020434)	5020434..5020745	-	312	WP_000897302.1	hypothetical protein	Toxin
HW370_RS24290 (5020975)	5020975..5021883	+	909	WP_001305059.1	alpha/beta hydrolase	-
HW370_RS24295 (5021947)	5021947..5022888	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HW370_RS24300 (5022933)	5022933..5023370	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HW370_RS24305 (5023367)	5023367..5024239	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HW370_RS24310 (5024233)	5024233..5024832	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T166256 WP_000897302.1 NZ_CP058222:c5020745-5020434 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T166256 NZ_CP074096:4786328-4786615 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT166256 WP_000356397.1 NZ_CP058222:c5020433-5020143 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT166256 NZ_CP074096:4786602-4786904 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: S1P416