Detailed information of TA system TA166250

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 4457264..4457676
• Replicon: chromosome
• Accession: NZ_CP058222
• Organism: Escherichia coli strain CFT073

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: HW370_RS21670
• Protein ID: WP_000132630.1
• Coordinates: 4457335..4457676 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4457264..4457340 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW370_RS21660 (4453876)	4453876..4455345	+	1470	WP_001315223.1	type I restriction-modification system subunit M	-
HW370_RS21665 (4455345)	4455345..4457114	+	1770	WP_000110076.1	restriction endonuclease subunit S	-
- (4457264)	4457264..4457340	-	77	NuclAT_6	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_6	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_6	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_6	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_7	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_7	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_7	-	Antitoxin
- (4457264)	4457264..4457340	-	77	NuclAT_7	-	Antitoxin
HW370_RS21670 (4457335)	4457335..4457676	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
HW370_RS21675 (4457723)	4457723..4458886	-	1164	WP_072202028.1	DUF1524 domain-containing protein	-
HW370_RS21680 (4458934)	4458934..4459816	-	883	Protein_4252	DUF262 domain-containing protein	-
HW370_RS21685 (4460222)	4460222..4461142	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
HW370_RS21690 (4461327)	4461327..4462607	+	1281	WP_024182263.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T166250 WP_000132630.1 NZ_CP058222:4457335-4457676 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T166250 NZ_CP074096:c3728731-3728363 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT166250 NZ_CP058222:c4457340-4457264 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin