Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2640188..2640750 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HW370_RS12595 Protein ID WP_000605675.1
Coordinates 2640188..2640466 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HW370_RS12600 Protein ID WP_000781370.1
Coordinates 2640466..2640750 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS12570 (2635774) 2635774..2636205 - 432 WP_000152307.1 peroxiredoxin OsmC -
HW370_RS12575 (2636550) 2636550..2636765 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HW370_RS12580 (2636867) 2636867..2637004 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HW370_RS12585 (2637161) 2637161..2638858 + 1698 WP_000433460.1 malate dehydrogenase -
HW370_RS12590 (2638992) 2638992..2640002 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HW370_RS12595 (2640188) 2640188..2640466 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HW370_RS12600 (2640466) 2640466..2640750 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HW370_RS12605 (2640976) 2640976..2641629 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HW370_RS12610 (2641622) 2641622..2642506 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HW370_RS12615 (2642519) 2642519..2645566 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T166244 WP_000605675.1 NZ_CP058222:2640188-2640466 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T166244 NZ_CP074096:c528434-528216 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT166244 WP_000781370.1 NZ_CP058222:2640466-2640750 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT166244 NZ_CP074096:c528836-528462 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References