Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2008032..2008534 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag HW370_RS09575 Protein ID WP_000767822.1
Coordinates 2008280..2008534 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HW370_RS09570 Protein ID WP_001259255.1
Coordinates 2008032..2008283 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS09545 (2003210) 2003210..2004277 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HW370_RS09550 (2004277) 2004277..2005347 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
HW370_RS09555 (2005344) 2005344..2006648 - 1305 WP_001297912.1 histidinol dehydrogenase -
HW370_RS09560 (2006654) 2006654..2007553 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HW370_RS09565 (2007699) 2007699..2007749 - 51 WP_001364200.1 his operon leader peptide -
HW370_RS09570 (2008032) 2008032..2008283 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HW370_RS09575 (2008280) 2008280..2008534 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HW370_RS09580 (2008617) 2008617..2009441 + 825 WP_000754733.1 SDR family oxidoreductase -
HW370_RS09585 (2009487) 2009487..2010416 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
HW370_RS09590 (2010631) 2010631..2010693 + 63 WP_010723108.1 membrane protein YoeI -
HW370_RS09595 (2010683) 2010683..2012041 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T166233 WP_000767822.1 NZ_CP058222:2008280-2008534 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T166233 NZ_CP074094:c3680272-3679997 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT166233 WP_001259255.1 NZ_CP058222:2008032-2008283 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT166233 NZ_CP074094:c3680535-3680275 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References