Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1263396..1264123 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HW370_RS06110 Protein ID WP_000547555.1
Coordinates 1263396..1263707 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HW370_RS06115 Protein ID WP_000126293.1
Coordinates 1263704..1264123 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS06080 (1258537) 1258537..1260246 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HW370_RS06085 (1260256) 1260256..1260798 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HW370_RS06090 (1260798) 1260798..1261565 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HW370_RS06095 (1261562) 1261562..1261972 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HW370_RS06100 (1261965) 1261965..1262435 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HW370_RS06105 (1262478) 1262478..1263233 + 756 WP_001305268.1 hypothetical protein -
HW370_RS06110 (1263396) 1263396..1263707 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HW370_RS06115 (1263704) 1263704..1264123 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HW370_RS06120 (1264215) 1264215..1264643 - 429 WP_000536064.1 hypothetical protein -
HW370_RS06125 (1265028) 1265028..1265555 + 528 WP_001078777.1 electron transport protein HydN -
HW370_RS06130 (1265708) 1265708..1267960 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T166232 WP_000547555.1 NZ_CP058222:1263396-1263707 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T166232 NZ_CP074094:c3206685-3206272 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT166232 WP_000126293.1 NZ_CP058222:1263704-1264123 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT166232 NZ_CP074094:c3206932-3206666 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References