Detailed information of TA system TA166229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 670582..671381
• Replicon: chromosome
• Accession: NZ_CP058222
• Organism: Escherichia coli strain CFT073

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: HW370_RS03305
• Protein ID: WP_000347252.1
• Coordinates: 670582..671046 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: HW370_RS03310
• Protein ID: WP_001296435.1
• Coordinates: 671046..671381 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW370_RS03275 (665583)	665583..666017	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HW370_RS03280 (666035)	666035..666913	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HW370_RS03285 (666903)	666903..667682	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HW370_RS03290 (667693)	667693..668166	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HW370_RS03295 (668189)	668189..669469	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HW370_RS03300 (669718)	669718..670527	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HW370_RS03305 (670582)	670582..671046	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HW370_RS03310 (671046)	671046..671381	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HW370_RS03315 (671530)	671530..673101	-	1572	WP_001273940.1	galactarate dehydratase	-
HW370_RS03320 (673476)	673476..674810	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
HW370_RS03325 (674826)	674826..675596	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T166229 WP_000347252.1 NZ_CP058222:c671046-670582 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T166229 NZ_CP074094:c1627001-1626717 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT166229 WP_001296435.1 NZ_CP058222:c671381-671046 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT166229 NZ_CP074094:c1627239-1626991 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5