Detailed information of TA system TA166227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4914425..4915027
• Replicon: chromosome
• Accession: NZ_CP058220
• Organism: Escherichia coli strain 83972

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HW371_RS23700
• Protein ID: WP_000897302.1
• Coordinates: 4914716..4915027 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW371_RS23695
• Protein ID: WP_000356397.1
• Coordinates: 4914425..4914715 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW371_RS23670 (4910498)	4910498..4911400	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HW371_RS23675 (4911397)	4911397..4912032	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HW371_RS23680 (4912029)	4912029..4912958	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HW371_RS23685 (4913174)	4913174..4913392	-	219	WP_001314326.1	CopG family transcriptional regulator	-
HW371_RS23690 (4913788)	4913788..4914066	-	279	WP_001296612.1	hypothetical protein	-
HW371_RS23695 (4914425)	4914425..4914715	-	291	WP_000356397.1	NadS family protein	Antitoxin
HW371_RS23700 (4914716)	4914716..4915027	-	312	WP_000897302.1	hypothetical protein	Toxin
HW371_RS23705 (4915257)	4915257..4916165	+	909	WP_001305059.1	alpha/beta hydrolase	-
HW371_RS23710 (4916229)	4916229..4917170	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HW371_RS23715 (4917215)	4917215..4917652	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HW371_RS23720 (4917649)	4917649..4918521	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HW371_RS23725 (4918515)	4918515..4919114	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T166227 WP_000897302.1 NZ_CP058220:c4915027-4914716 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T166227 NZ_CP074094:c339660-339385 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT166227 WP_000356397.1 NZ_CP058220:c4914715-4914425 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT166227 NZ_CP074094:339395-339817 [Salmonella enterica subsp. enterica serovar Typhimurium]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: S1P416