Detailed information of TA system TA166221

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 4319691..4320103
• Replicon: chromosome
• Accession: NZ_CP058220
• Organism: Escherichia coli strain 83972

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: HW371_RS20915
• Protein ID: WP_000132630.1
• Coordinates: 4319762..4320103 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4319691..4319767 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW371_RS20905 (4316303)	4316303..4317772	+	1470	WP_001315223.1	type I restriction-modification system subunit M	-
HW371_RS20910 (4317772)	4317772..4319541	+	1770	WP_000110076.1	restriction endonuclease subunit S	-
- (4319691)	4319691..4319767	-	77	NuclAT_6	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_6	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_6	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_6	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_7	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_7	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_7	-	Antitoxin
- (4319691)	4319691..4319767	-	77	NuclAT_7	-	Antitoxin
HW371_RS20915 (4319762)	4319762..4320103	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
HW371_RS20920 (4320150)	4320150..4321313	-	1164	WP_072202028.1	DUF1524 domain-containing protein	-
HW371_RS20925 (4321361)	4321361..4322243	-	883	Protein_4098	DUF262 domain-containing protein	-
HW371_RS20930 (4322649)	4322649..4323569	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
HW371_RS20935 (4323754)	4323754..4325034	+	1281	WP_001304526.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T166221 WP_000132630.1 NZ_CP058220:4319762-4320103 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T166221 NZ_CP074092:4241649-4241960 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT166221 NZ_CP058220:c4319767-4319691 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin