Detailed information of TA system TA166194

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4189310..4189568
• Replicon: chromosome
• Accession: NZ_CP058217
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: hokC
• Uniprot ID: S1NX00
• Locus tag: HW372_RS20060
• Protein ID: WP_000809168.1
• Coordinates: 4189416..4189568 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokC
Locus tag	-
Coordinates	4189310..4189367 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW372_RS20045	4185139..4186398	-	1260	WP_000494928.1	hypothetical protein	-
HW372_RS20050	4186527..4188020	-	1494	WP_001443162.1	sulfatase-like hydrolase/transferase	-
HW372_RS20055	4188040..4188801	-	762	WP_001274832.1	outer membrane protein OmpK	-
-	4189310..4189367	-	58	-	-	Antitoxin
HW372_RS20060	4189416..4189568	+	153	WP_000809168.1	type I toxin-antitoxin system toxin MokC	Toxin
HW372_RS20065	4189673..4190803	-	1131	WP_001118465.1	molecular chaperone DnaJ	-
HW372_RS20070	4190892..4192808	-	1917	WP_000516135.1	molecular chaperone DnaK	-
HW372_RS20075	4193180..4193584	+	405	WP_000843687.1	DUF2541 family protein	-
HW372_RS20080	4193610..4194323	+	714	WP_001102391.1	acidic protein MsyB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5501.57 Da
• Isoelectric Point: 7.1312

>T166194 WP_000809168.1 NZ_CP058217:4189416-4189568 [Escherichia coli Nissle 1917]
MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE

Nucleotide

• Length: 153 bp

>T166194 NZ_CP074087:331139-331507 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT166194 NZ_CP058217:c4189367-4189310 [Escherichia coli Nissle 1917]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: S1NX00

Antitoxin