Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2576190..2576752 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HW372_RS12240 Protein ID WP_000605675.1
Coordinates 2576190..2576468 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HW372_RS12245 Protein ID WP_000781370.1
Coordinates 2576468..2576752 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS12215 (2571776) 2571776..2572207 - 432 WP_000152307.1 peroxiredoxin OsmC -
HW372_RS12220 (2572552) 2572552..2572767 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HW372_RS12225 (2572869) 2572869..2573006 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HW372_RS12230 (2573163) 2573163..2574860 + 1698 WP_000433460.1 malate dehydrogenase -
HW372_RS12235 (2574994) 2574994..2576004 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HW372_RS12240 (2576190) 2576190..2576468 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HW372_RS12245 (2576468) 2576468..2576752 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HW372_RS12250 (2576981) 2576981..2577634 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HW372_RS12255 (2577627) 2577627..2578511 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HW372_RS12260 (2578524) 2578524..2581571 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T166189 WP_000605675.1 NZ_CP058217:2576190-2576468 [Escherichia coli Nissle 1917]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T166189 NZ_CP074076:c620065-619913 [Xanthomonas oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT166189 WP_000781370.1 NZ_CP058217:2576468-2576752 [Escherichia coli Nissle 1917]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT166189 NZ_CP074076:c619849-619715 [Xanthomonas oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References